No. If there are macro scripts for those M codes, then they are run instead of the stock actions. So if you have the "example" scripts, all it will do is print "Spindle Clockwise", etc... in the message area.
The stock action is to use the spindle delays in the config and turn the signals on/off. If you want more control, write a M3/M5 script. For instance, my VFD can tell me if it is at speed or at zero. I can then make the M3/M4/M5 scripts wait on the speed or make sure the spindle is stopped before moving on.
Steve