Hi, Iqchallengedd
Things can get out of Whack with Mach3's driver sometimes, Probably best to remove it and reinstall at this point.
Open Device Manager, Rt click on Mach3 Driver, Select "Uninstall" Mach3 Driver, Yes.
Return to Mach3 Folder, C:\Mach3 and "Run" the "Driver Test", It will return with the "Driver Installed", Reboot/Re-start Computer Required.
Restart and run Mach3, From time to time this has been the only solution for Me.
You now after Using, Your Fore-Head to Drive 16 Penny Nail's in the Wall a few times.
Hope this Helps, Chip