When Mach3 is running a program, you should not do ANYTHING on the PC, except click Feedhold, or Stop.
Running other programs will almost always cause issues. Even clicking in Mach3's toolpath can cause issues on some PC's.
If you want to run V-Carve Pro while the machine is running, get an external motion controller like a Smoothstepper. ut I'd still recommend using a separate design PC.