In Motor tuning you need to set a Steps per unit for each axis you are using. This is a calculated value and will depend on the hardware you have.

As an example a machine may have 1.8 degree steppers(200 steps per rev) and use 10 micro step drives, the motor is coupled direct to the ballscrew which is 0.2 pitch.

With these figures you can work out the steps per unit, it would take 200 steps to rotate the motor one turn but because there is microstepping it would then be 200 x 10usteps = 2000 to rotate 1 turn. One turn of the motor would turn the ballscrew one turn and because it is 0.2" pitch it would take 5 turns to move one inch or one unit. So 2000 x 5 would equal 10,000steps per unit.

So if you know the components you can work out what the steps per unit should be.

Hood