If the image is mirrored left to right, your X axis is backwards. If mirrored top to bottom, the Y is backwards. Can you post some pics of the machine, as it would help explain things better.
As for the steps, 1/2-10 5 start screws are 400 steps per revolution with full stepping, 800 for half step, 1600 for 1/4 step, 3200 for 1/8 step, or 4000 for 1/10 step (Gecko). What are your drives set at?