Hi, Barry
May have a bad xml file.
Look in C:\Mach3\xmlbackups folder, It has backup xml's, Sort it by Date, Make a copy of the one that is just before the date you started having trouble.
Past it in C:\Mach3\ folder, Then re name it th Mach3Test.xml and load it, Give it a try.
With the boards your using there are a lot of special settings, It's probably the encoder interface board's settings your missing.
Thanks, Chip