Hi,
unfamiliar with that fault. Mach3 uses Visual Basic, or rather a subset of it called C(ypress) E(nable), and I would guess that the VB interpreter is not loading or working.

I have not used Mach3 for nearly eight years, I use Mach4, and mach4 does not rely on VB but rather Lua.

Craig

There are a few references to this fault arising both here and in other places on the web, but no fix I'm afraid!  I can't see anywhere in the Mach 3 folder structure where the interpreter would be, but I know that it's there and works in Win10 as macros work on my lathe and mill both using Win10 over USB.  All I can suggest is that you reinstall Mach 3 again, getting a fresh copy from the NFS download page, and try again.