Sounds like progress

Initially check that you have Mach3 set in mm Units (Config. / Select Native units).
Now you need to check the leadscrew pitch of your Z axis – I am assuming it is 4mm (one complete revolution of the lead screw moves the axis 4mm).
Steps per unit are calculated as follows…
Your steppers have 200 whole steps per revolution & at 1/8th microstepping that makes it 1600 steps per revolution ( 200*8 ). If your Z axis moves 4mm in one complete revolution of the leadscrew then to move it 1mm or 1 unit would require 400 steps (1600/4). This would make the steps per unit 400 in Motor Tuning.
You should repeat this measurement and calculation for the X and Y axis just to check that they are to be set at 400 steps per unit.
Next is axis direction…
Standing at the front of the machine, if you press the up arrow key on the keyboard the gantry should move away from you. If it moves in the wrong direction then change the Direction Low Active state for the Y axis in Config. / Ports & Pins / Motor Outputs and apply the change.
If you now press the left arrow key the carriage should move to the left. If it moves in the wrong direction then change the Direction Low Active state for the X axis in and apply the change.
If you press the Page Up key the Z axis should move upwards. If it moves in the wrong direction then change the Direction Low Active state for the Z axis in and apply the change.
One step at a time, we will get there

Tweakie.