You need to assign pin numbers for any pins you'll be using.

I really appreciate your help Gerry.  I will see how it goes.  Thanks

Gerry, I assigned pins and port 1, for X,Y,Z in Motor Configuration and enabled them.  I assume to get the steppers to move X,Y table and Z that I must also assign pins and possibly ports in either Mach3 Input or Output.  Do not know what to do after  X,Y,Z in Motor Configuration.  Please assist me.

Is anyone using the UC100 with Mach3?