In Screen 4, configure the button to execute VB Script.
Then, in Mach3, go to the Operator menu, and select "Edit Button Script". Click on the button, and enter the code I posted in the script editor that opens. Go to File > Save in the VB editor, then close it.
When you click the button, it will toggle the LED on and off.
I'm not sure if 1000 is a valid User LED number. You might ant to use a higher vale, over 1200 or so?