Have you defined your "e-stop" input and set it to be enabled.
No e-stop defined, nothing will move.. you can "frig" the e-stop by defining it and then setting it to either active high or low and that will perimiantly clear the e-stop (see my note below regarding mushroom buttons)
I'd suggest you go into you C:/Mach3 folder and copy and paste you XML file so we can have a look at what you've setup and what you may not have setup.
the XML file defines all of your settings.... it is normally called by the same name that your mach3 profile is (for instance MachMill / Plasma etc etc). you can open the file up using notepad (although it will look like a long read trying to order everything.... there is an SML viewer listed on here somewhere.... I personally use the Microsoft XML editor (available free)... but normally just load your profile into mach and run through all the settings.
It would be helpful to know something about your machine setup (i.e. what leadscrews you are using (their pitch), and what settings you have for microstepping on your drives, what type of machine you are running .... mill, plasma, etc ... do you have an e-stop (essential if you ask me! [can't beat a mushroom button in a panic]), do you have home switches, limit switches etc etc.
It is sooo much easier looking at an XML file than just asking questions and batting things back & forth.
Rob