As long as I don't load g code from mill wizard. All moves are accurate one inch travel on axis = one inch on Mach3 screen dro.
Did you check the Native Units?
If you configure your machine to accurately move in inches, but the Native units are set to mm's, then any g-code without a G20 will move the correct distance in inches, but if there's a G20, it will move 25.4x more than it's supposed to, which is what you said in your first post.