Before your second post, I KNEW you had a smoothstepper, that's the error you get trying to restart Mach3 after the SS has lost communication.
The SS lost communication with the computer. It happens and there have been many sort-of solutions here and on the warp9 forum. Look there or here for possible things to try, or perhaps one of the experts will pop in and point you. I don't remember where all this talk took place. It was mostly to do with grounding, but also powering the SS from an external 5v power supply, plugging the SS into a powered USB hub instead of the pc directly, and other solutions, none of which were really definitive!! Search around and try some of these things, different USB cable, power from external 5v supply, USB hub, cutting the outside metal shield off the SS end of the USB cable (carefully!!), unsoldering some stuff on the SS itself (I never went there!)