Can you go into the Mach3 folder and run the Driver test and see how that goes. Actually sounds like it could be a low volt parallel port but if you could list your hardware( Drives, motors, breakout etc) it will help. Also if you could attach your xml file we might be able to find a problem, it will be the xml forthe profile you use, ie if you use Mach3 Mill its that xml, if you use a custom profile its that one.
Hood