I have installed a second PCI printer port on the mother board and did a printer test and works good.  My problem is that Mach3 will not respond to any control from that port.  In control panel the driver has a starting address of E010 I entered it in the Mach setup screen. checked port #2 enabled and checked use 2 -9 as inputs.  Could it be the address is wrong? I thought printer port 2 address is 278.

It could be that the port is not a real port but just emulating a port, another possibilty is that it is not capable of being set for inputs on 2-9. Check by setting an input in ports and pins to port 2 pin 10 and then watch the LED for that input on the diagnostics page and see if it reacts when you short pin 10 to a gnd pin.
Hood

You can confirm the address of your second printer port from:- control panel/hardware/device manager/ports/properties/resources.

Tweakie.