I am trying to build a latch circuit that is associated with the reset button.  I have a conveyor with 20 zones, each is controlled through a relay output on the DL06.  I have manual buttons set to turn each zone on and off.  I have it set that it will only function with the reset actuated, but I need it to drop the output if the reset or the estop is hit.  Should I do this in VB or with a brain, or in the PLC logic?

You could do it any of the above.

1). In Brian have your Estop as your input, go through a "Invert" lobe, then to your output you want to drop.
2). In Macro pump you can do the same as above watching LED800, to unset your output
3). You can also use one of your Enables 1, 2 or 3 as a control bit in your PLC When Mach is active (not reset) the Enable will toggle your NO, or NC control bit in what ever rung you have going to your convayor Reley/Enable.

Scott