Short Update:
Changed all power supplies to 36 Volt and changed a setting in the ESS Plugin.
Insted of telling Mach4 Motor 3 is Motor 1 Slave, I mached them in the ESS Plugin and tell Mach4 only you have 3 Motors, Motor 0,1,2.
The problem with the steps/unit with the Y Axis is now gone.
I can't tell why, but I guess if you say in Mach4 under Mapping A is slave of Y and in the motor settings motor 0-3 enabled and tuned, maybe there was I did something wrong. Now all work the same speed with the same pules.
Tomorrow, will test to mill a new part and will give a feedback.
Oli