Hi,
conventional direction is downwards Z decreases.
At Z=5, say, the Z axis is 5 units above the surface of the material. If the Gcode calls for Z=-0.1, its intent is to cut 0.1 units off the top surface.
Does your Z axis DRO decrease as you lower the spindle? If not you need to change the Z axis direction. Config/Ports and Pins/Motor Outputs check (uncheck)
<Dir Active Low>.
Craig