So, after getting PC #2 to come on, yeah it's been sitting for a year lol, we installed Mach3 and ran through the settings and voila! the motors are turning! So now the determination is that the new card we put in our main PC is no good. Going to go ahead and save the settings and move them over to the main PC to double check but we're fairly certain that it won't work. Thank you for your help Brett! Troubleshooting can be a major pain and it certainly makes you rack your brain. Just glad that we had another PC sitting around to work with. We had that one laying around because there was something wrong with it, but we diagnosed it to be the video card and just removed it and used the onboard.