Do not adjust the tuning unless you are running the FULL travel. Go get a long ruler. I got a 72 inch starret aluminum straight edge with numbers for about $25.
Then chuck a needle or a pointy thing into the spindle and go to zero, then to the extreme other edge, then tune till you hit that far end. The remaining error is the maximum error you will have. Which means that if you only travel 1 inch that it's 1/72 of that error.
Then route a large square/angle into mdf. If that 's not straight, then your gantry is not aligned properly (left right side). Or something is loose and introduces backlash. One of the machines I build had backlash because a screw hit the and of the thread and did not clamp down fully. So... with larger forces in curves, that darn thing slipped back and force 0.01- .03..... Backlash where there should never be any. So don't take things for granted. Check the mechanics and tune with the largest moved you can make. You can verify with a dial, but do not think that you will be achieving the best precision only running short distances.
Mach3 does cut straight lines and round circles. I had to make that painful experience as well
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Lemo