It looks like disabling ACPI also screws up the plug and play or device discovery, I would think that would cause more problems.
In most cases, ACPI prevents Mach3 from running properly, and changing to Standard will usually fix this. Both PC's I have will not run Mach3 in ACPI mode, but work fine in Standard mode.
Well the 400 seems like a lot of extra wiring and work for a benefit I do not need.
The UC400ETH require no more wiring than a UC100. It just requires a ribbon cable to the G540 instead of a parallel cable. About $5. And an ethernet cable instead of USB. $2.
Ethernet provides a more reliable connection, and is more immune to noise issues.
Mach3 runs just fine on my Lenovo laptop with ACPI and I ran an hour long very intensive VCarve gcode file without any issues what so ever. I even bumped the feed rate up 200% and the computer CPU was only using 30%. The 400 would require mounting inside the power/controller box and adding a power source which I could do. I will switch over to Standard to see if changes things.
So does Mach3 set the pulse frequency as needed?
Did I mention that I am / was a master electrician and specialized in HVAC/R controls. Also have been working with and building computers as a hobby since about 1980, and also a licensed ham back when you had to know something to get your call sign.