I will tell you what happened in my case:
The pokeys was working fine, with the last PoKeys Software, but Matt and I found a INvert Output bug in the software. He sent me a new PoKeys Software that was to fix the firmware bug. When I installed it, the software could no longer "see" the pokeys, it keep telling me that their was NOT one conncected and did I want to goto demo mode. The Red LED would come on fine, but, when you would plug it in, windows would not see it as a USB device, even when looking at it under device manager. I tried the Reset procedure and several other things under Matts guidance none caused the PoKeys Software to be able to connect to the device, nor would windows see it or connect to it as a USB device.
scott